細(xì)胞循環(huán)PCR芯片可用于研究參與細(xì)胞循環(huán)調(diào)控的84個(gè)關(guān)鍵基因的表達(dá)。這一芯片包含了參與細(xì)胞周期、周期轉(zhuǎn)換、DNA復(fù)制，以及細(xì)胞循環(huán)過程中正調(diào)控和負(fù)調(diào)控的基因。通過實(shí)時(shí)定量PCR的方法，研究者即能夠利用該芯片簡(jiǎn)單可靠地同時(shí)檢測(cè)細(xì)胞循環(huán)相關(guān)基因的表達(dá)。
G1 Phase and G1/S Transition: ANAPC2, CCND1, CCNE1, CDC25A, CDC34, CDK4, CDK6, CDKN1B (p27KIP1), CDKN3, CUL1, CUL2, CUL3, E2F1, SKP2. S Phase and DNA Replication: ABL1, CDC6, MCM2, MCM3, MCM4, MCM5, WEE1. G2 Phase and G2/M Transition: ANAPC2, BCCIP, BIRC5, CCNA2, CCNB1, CCNG1, CCNH, CCNT1, CDC25A, CDK5R1, CDK5RAP1, CDK7, CDKN3, CKS1B, CKS2, GTSE1, KPNA2, MNAT1, SERTAD1. M Phase: AURKB, CCNB2, CCNF, CDC25C, CDC6, CDK1 (CDC2), CDC16, CDC20, MRE11A, RAD51, STMN1. Cell Cycle Checkpoint and Cell Cycle Arrest: ATM, ATR, BRCA1, BRCA2, CASP3, CCNA2, CCNG2, CDC25A, CDC25C, CDC34, CDK1 (CDC2), CDK2, CDKN1A (p21CIP1/WAF1), CDKN1B (p27KIP1), CDKN2A (p16INK4), CDKN2B (p15INK4B), CDKN3, CHEK1, CHEK2 (RAD53), CUL1, CUL2, CUL3, GADD45A, HUS1, KNTC1, MAD2L1, MAD2L2, MDM2, NBN (NBS1), RAD1, RAD17, RAD9A, RB1, RBBP8, TP53, WEE1. Regulation of Cell Cycle: ABL1, ANAPC2, ATR, AURKA, BCCIP, BCL2, BRCA2, CCNB1, CCNB2, CCNC, CCND1, CCND2, CCND3, CCNE1, CCNF, CCNH, CCNT1, CDC16, CDC20, CDC25C, CDC6, CDK1 (CDC2), CDK2, CDK4, CDK5R1, CDK6, CDK7, CDK8, CDKN1A (p21CIP1/WAF1), CDKN1B (p27KIP1), CKS1B, E2F1, E2F4, GADD45A, KNTC1, MKI67, RAD9A, RB1, SKP2, TFDP1, TFDP2, WEE1. Negative Regulation of Cell Cycle: ATM, BRCA1, CASP3, CDKN2B (p15INK4B), RBL1, RBL2, TP53. |
